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LigandTracer Instruments rely on a simple and robust technology for detecting protein-cell interactions in real-time. The key function is the possibility to detect interactions during incubation. This is made possible by including one active area (the seeded"target cells) and one in situ reference area in the cell-dish. The cell-dish is then placed on a inclined, slowly rotating support. Liquid containing labeled protein is added to the dish and accumulates in the bottom part. Each revolution the cells will get in contact with the liquid. The detector is mounted over the upper part, collimated to read only the part of the dish that is essentially liquid-free.

When running, the activity is measured several times per revoution. If the protein binds to the cells, clear peaks will be seen in the graph. The peak height from each revolution is automatically extracted and can be followed over time (as in uptake/retention measurements). Alternatively, peak heights obtained from different concentrations of radiolabeled protein can be used to calculate the affinity of the interaction.

LigandTracer is currently adapted to measurements of how radiolabeled proteins interact with cell-surface structures or receptors. The technology itself is not dependent on detection of radiolabels, but works with other labeling technologies as well (e.g. fluorescent markers).

Benefits of LigandTracer 
When compared to manual measurements, LigandTracer has four main benefits:

* Binding of a protein to a cell can be followed in real-time during minutes, hours, or days
* Binders with rapid dissociation rates can be correctly characterized
* There is no need to wash cell-dishes
* LigandTracer saves reagents and labor time

Read more

LigandTracer for interaction analysis, Technology Note

Scientific references